Quantitative HPLC Analysis of Plasma Amino Acids as Orthophthaldialdehyde/Ethanethiol Derivatives
- 31 December 1981
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 5 (12) , 2369-2393
- https://doi.org/10.1080/01483918208067641
Abstract
A reverse phase high performance liquid chromatographic [HPLC] method for the analysis of [rat] plasma amino acids is described. The method employs pre-column derivatization with o-phthaldialdehyde using ethanethiol as the reducing agent. The analysis shows good linearity and reproducibility. An average overall difference of 12% was seen for results obtained by the HPLC method vs. those obtained with an amino acid analyzer. The chromatographic parameters of buffer concentration and column temperature were also examined.Keywords
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