Separation of Two Hemolysins from Aeromonas hydrophila by Isoelectric Focusing
- 1 October 1971
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 4 (4) , 503-505
- https://doi.org/10.1128/iai.4.4.503-505.1971
Abstract
Two hemolytic proteins (hemolysin I and II) with isoelectric points of 5.5 and 4.3 were purified by isoelectric focusing from the culture supernatant fluid of Aeromonas hydrophila . The purified hemolysins were unstable after dialysis and upon heating at 37 C and were inactivated by proteolytic enzymes. The two hemolysins were lethal for mice in a dose of 10 μg (hemolysin I) and 3 μg (hemolysin II) and showed similar hemolytic spectra on red cells of different species. Both were also cytotoxic for HeLa cells and human fibroblasts and gave dermonecrosis in rabbits.Keywords
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