Studies on transfer ribonucleic acids and related compounds. XXV. Synthesis of E. coli tRNAfMet 5'-terminal tetranucleotide C-G-C-Gp and its sequence analog U-G-C-Gp.

Abstract
A tetranucleotide C-G-C-Gp (cytidylyl-(3''-5'')-guanylyl-(3''-5'')-cytidylyl-(3''-5'')-guanosine 3''-phosphate) was synthesized by condensation of (Bz)bzC(Bz)-ibG(Bz)p with C(Bz)-ibG(Bz)pNHC6H4S(O)CH3, using 2,4,6-triisopropylbenzenesulfonyl chloride. This sequence corresponds to the Escherichia coli tRNAfMet 5''-terminus. A base substituted analog U-G-C-Gp was also synthesized by a similar procedure to that used for C-G-C-Gp. These tetranucleotides were characterized by enzymic hydrolysis.