An Alternative to SH2 Domains for Binding Tyrosine-Phosphorylated Proteins
- 16 December 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 266 (5192) , 1862-1865
- https://doi.org/10.1126/science.7527937
Abstract
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that participate in signaling by growth factors and oncogenes. A protein domain was identified that bound specifically to the tyrosine-phosphorylated form of its target protein but differs from known SH2 sequences. Phosphotyrosine-binding (PTB) domains were found in two proteins: SHC, a protein implicated in signaling through Ras; and SCK, encoded by a previously uncharacterized gene. The PTB domain of SHC specifically bound to a tyrosine-phosphorylated 145-kilodalton protein. PTB domains are an alternative to SH2 domains for specifically recruiting tyrosine-phosphorylated proteins into signaling complexes and are likely to take part in signaling by many growth factors.Keywords
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