Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATP‐dependent K+ channel, ROMK6 (Kir1.1 f)
- 2 December 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 399 (1-2) , 122-126
- https://doi.org/10.1016/s0014-5793(96)01302-6
Abstract
We have identified from rat kidney a novel isoform of ROMK/Kir1.1, designated ROMK6/Kir1.1f. ROMK6 was nearly identical to ROMK1, but possessed an 122‐bp insertion in the 5′ region. Its deduced amino acid sequence was shorter by 19 amino acids than that of ROMK1 in the amino‐terminus. Unlike other previously reported ROMK isoforms, ROMK6 mRNA was ubiquitously expressed in various tissues, including kidney, brain, heart, liver, pancreas and skeletal muscle. Xenopus oocytes injected with ROMK6 cRNA expressed a Ba2+‐sensitive weakly inwardly rectifying K+ current. These results indicate that ROMK6 is a novel functional K+ channel and might be involved in K+ secretion in various tissues.Keywords
This publication has 16 references indexed in Scilit:
- Promiscuous coupling between the sulphonylurea receptor and inwardly rectifying potassium channelsNature, 1996
- Renal potassium channels: An overviewKidney International, 1995
- The inward rectifier potassium channel familyCurrent Opinion in Neurobiology, 1995
- Intracellular H+ inhibits a cloned rat kidney outer medulla K+ channel expressed in Xenopus oocytesAmerican Journal of Physiology-Cell Physiology, 1995
- Expression of the cystic fibrosis gene in adult human lung.Journal of Clinical Investigation, 1994
- Localization of cystic fibrosis transmembrane conductance regulator mRNA in the human gastrointestinal tract by in situ hybridization.Journal of Clinical Investigation, 1994
- Cloning and expression of an inwardly rectifying ATP-regulated potassium channelNature, 1993
- Renal Potassium Channels and Their RegulationAnnual Review of Physiology, 1992
- Structural features in eukaryotic mRNAs that modulate the initiation of translation.Journal of Biological Chemistry, 1991