A SENSITIVE METHOD FOR MEASUREMENT OF AMINO ACYL RIBONUCLEIC ACID SYNTHETASE ACTIVITIES
- 1 May 1962
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 40 (5) , 653-666
- https://doi.org/10.1139/o62-075
Abstract
A sensitive method for the measurement of amino acyl RNA synthetase activities (amino acid activating enzymes) is described. The method is based on measurements of the rates of labelling of soluble ribonucleic acid with14C-amino acids. Determinations of α-glutamyl-RNA, glutaminyl-RNA, and glycyl-RNA synthetase activities in the "pH 5 enzymes" fractions from rat liver and mouse Ehrlich ascites carcinoma cells have been made.Keywords
This publication has 26 references indexed in Scilit:
- A SOLUBLE RIBONUCLEIC ACID INTERMEDIATE IN PROTEIN SYNTHESISPublished by Elsevier ,2021
- ENZYMATIC CARBOXYL ACTIVATION OF AMINO ACIDSPublished by Elsevier ,2021
- The reactions of amino acids with soluble ribonucleic acid from guinea-pig mammary cellsBiochemical Journal, 1959
- Purification and Properties of Tyrosine-activating Enzyme of Hog PancreasJournal of Biological Chemistry, 1958
- Amino Acid Activation in Plant TissuesJournal of Biological Chemistry, 1958
- INCORPORATION OF C14-AMINO ACIDS INTO RIBONUCLEOPROTEIN PARTICLES FROM THE EHRLICH MOUSE ASCITES TUMORJournal of Biological Chemistry, 1957
- A new method for the isolation of ribonucleic acids from mammalian tissuesBiochemical Journal, 1956
- THE CHEMISTRY OF INSECT HEMOLYMPHThe Journal of general physiology, 1956
- Studies on the Enzymatic Synthesis of GlutaminePlant Physiology, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951