The Culture of Tissue Cells in Clots Formed from Purified Bovine Fibrinogen and Thrombin.
- 1 June 1947
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 65 (2) , 309-314
- https://doi.org/10.3181/00379727-65-15945
Abstract
Expts. using clots formed from purified and sterilized thrombin and fibrinogen fractions of bovine blood plasma showed these clots to be equal to those from whole chick plasma for the cultivation of such tissue cells as rat fibroblasts, rat myoblasts, Jensen rat sarcoma cells, and chick fibroblasts. The use of these prepns. has the advantage over whole chick plasma in that storage of the purified reagents is practical for several wks., and the prepns. are sterile.Keywords
This publication has 7 references indexed in Scilit:
- Preparation and Properties of Serum and Plasma Proteins. IV. A System for the Separation into Fractions of the Protein and Lipoprotein Components of Biological Tissues and Fluids1a,b,c,dJournal of the American Chemical Society, 1946
- A STUDY OF TISSUE CULTURE CELLS BY ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1945
- CHEMICAL, CLINICAL, AND IMMUNOLOGICAL STUDIES ON THE PRODUCTS OF HUMAN PLASMA FRACTIONATION. III. AMINO ACID COMPOSITION OF PLASMA PROTEINS 1Journal of Clinical Investigation, 1944
- Preparation and Properties of Serum and Plasma Proteins. III. Size and Charge of Proteins Separating upon Equilibration across Membranes with Ethanol—Water Mixtures of Controlled pH, Ionic Strength and TemperatureJournal of the American Chemical Society, 1940
- Preparation and Properties of Serum and Plasma Proteins. II. Crystallization of a Carbohydrate-Containing Albumin from Horse SerumJournal of the American Chemical Society, 1940
- Preparation and Properties of Serum and Plasma Proteins. I. Size and Charge of Proteins Separating upon Equilibration across Membranes with Ammonium Sulfate Solutions of Controlled pH, Ionic Strength and TemperatureJournal of the American Chemical Society, 1940
- THE FUNDAMENTAL PROPERTIES OF THE FIBROBLAST AND THE MACROPHAGEThe Journal of Experimental Medicine, 1928