Fractionation and determination of the carbohydrate components from microbial products

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Abstract
A routine procedure was developed which enabled a complete quantitative analysis of the carbohydrate components of microbial products to be achieved within a few days. Using the procedure, the complete carbohydrate composition of two yeasts grown on hydrocarbons and a filamentous fungus grown on carbohydrate waste was determined. Glucose was the predominant sugar contained in the polysaccharide fractions from all samples. Mannose was present to a lesser extent and glucosamine was present in trace amounts. Galactose was found in trace amounts in one of the yeasts but was found in slightly higher amounts in the fungus grown on carbohydrate. Carbohydrates accounted for 22.4‐23.6% of the dry matter of hydrocarbon grown yeasts and 21.7% for the filamentous fungus.