Immunomagnetic Positive Selection and Colony Culture of CD34+ Cells from Blood
- 1 January 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Hematotherapy
- Vol. 2 (2) , 247-250
- https://doi.org/10.1089/scd.1.1993.2.247
Abstract
Immunomagnetic separation has been used to enrich CD34-positive cells in umbilical cord blood. Cell purities were increased from 0.59% preseparation to 92.7% postseparation (n = 16) with a mean yield of 75.7%. CFU were enriched 127 fold by immunomagnetic separation. Addition of combinations of recombinant growth factors resulted in cloning efficiencies of greater than 50%.Keywords
This publication has 2 references indexed in Scilit:
- Design of Large-Scale Separation Systems for Positive and Negative Immunomagnetic Selection of Cells Using Superparamagnetic MicrospheresJournal of Hematotherapy, 1992
- Hematopoietic Reconstitution in a Patient with Fanconi's Anemia by Means of Umbilical-Cord Blood from an HLA-Identical SiblingNew England Journal of Medicine, 1989