INDUCTION OF IgG BY LIPID A IN THE NEWBORN MOUSE

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Abstract
IgG of paternal allotype first becomes detectable in the serum of (BALB/c x C57BL/6)F1 mice between day 12 and 14 after birth and reaches adult levels at an age of 5 wk. Since in mice there is a transfer of maternal IgG molecules through the placenta and via milk, F1 heterozygous at the allotype locus were used and the concentrations of IgG with paternal allotype were measured. This was done by a sensitive method capable of detecting IgG concentrations as low as 5 x 10–4 of normal adult serum levels. It is based on the quantitative inhibition of allotype-specific facilitation of hemolysis.