Genome Typing of Three Serotypes of Porcine Adenovirus

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Abstract
Porcine enteric adenovirus serotypes 1–3, previously shown to be distinct antigenically, were purified by density gradient centrifugation, and the DNA was extracted. Fragments generated by digestion with restriction endonucleases BanMl, EcoRλ, Hindlll, and PaeKΙ were separated by electrophoresis and compared with human adenovirus type 2 as a control. The results demonstrated that the three porcine viruses were closely related to each other, although distinguishable, and distinct by restriction endonuclease analysis from human, murine, canine, bovine, and fowl adenovirus.