Fractionation of Human Milk Oligosaccharides by High-Performance Liquid Chromatography

Abstract
An ion-exchange chromatographic system was used to isolate several human milk oligosaccharides, the elution being carried out with a linear gradient of a sodium borate buffer. Lacto-N-tetraose, lacto-N-neotetraose, lacto-N-fucopentaose I, II and II, lacto-N-difucohexaose I and 2''-.alpha.-fucosyllactose can be separated by this method.

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