Formation of lipid—protein bilayers by micropipette guided contact of two monolayers
- 21 February 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 152 (2) , 187-190
- https://doi.org/10.1016/0014-5793(83)80376-7
Abstract
Bilayers of a few μm2 area were formed from lipid‐protein vesicles. First, two monolayers are generated from vesicles, which then are brought into local contact by use of a micro‐pipette to form a bilayer. Membrane channels of Matrix—Protein and of Colicin A exhibit normal properties in these bilayers. Apart from avoiding solvents completely and of using characterized vesicles as starting materials there are two main virtues to this strategy: (1) Current resolution is very high, a few tenths of a pA at 150 μs time resolution; and (2) transport processes can be studied in dependence of surface pressure between 28 and 48 mN/m.Keywords
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