Deletion ofSrcHomology 3 Domain Results in Constitutive Activation of Tec Protein-Tyrosine Kinase
Open Access
- 1 November 1996
- journal article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 87 (11) , 1106-1110
- https://doi.org/10.1111/j.1349-7006.1996.tb03118.x
Abstract
Tec protein‐tyrosine kinase (PTK) is the prototype of a new subfamily of non‐receptor type PTKs, and is abundantly expressed in hematopoietic tissues. We have revealed that Tec is inducibly tyrosine‐phosphorylated and activated by stimulation with a wide range of cytokines. To get more insight into the signaling mechanism through Tec, we have generated a constitutively active form of Tec PTK. Deletion of the Src homology (SH) 3 domain gave rise to a hyperphosphorylated and activated Tec kinase (TecΔSH3). The activity of TecΔSH3 was confirmed in 293 cells, as well as in cytokine‐dependent hematopoietic cells (BA/F3). TecΔSH3 should be a useful tool to study the in vivo substrates of Tec PTK.Keywords
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