MODIFIED BENZIDINE TEST FOR THE DETECTION OF CYTOCHROME-CONTAINING RESPIRATORY SYSTEMS IN MICROORGANISMS

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Abstract
The benzidine test for Fe-porphyrin compounds has been modified and adapted so that it is suitable for the routine detection of cytochrome-containing respiratory systems in microorganisms [organisms used included: Salmonella, Paracolobactrum and Desulfo -vibrio strains, Microbacterium flavum, M. lacticum, Pneumococcus, Corynebacterium, Shigella dysenteriae, Clostridium, Erysipelothrix, Propionibacterium, Escherichia, Proteus, Aerobacter, Shigella, Staphylococcus aureus and Lactobacillus plantarum.] It has been shown to be much more suitable than the catalase test for the delin-eation of the lactic acid groups of bacteria and their differentiation from morphologically similar bacteria. Also, it would appear to be of value in studies on the physiological relationship of other groups or genera of microorganisms.