Identification by a multiplex PCR‐based assay ofSalmonellaTyphimurium andSalmonellaEnteritidis strains from environmental swabs of poultry houses

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Abstract
A multiplex‐PCR‐based assay (m‐PCR) was developed for the detection of Salmonella and for the identification of the two serotypes Enteritidis and Typhimurium. Three sets of primers selected from different genomic sequences amplified a 429 bp fragment specific for the genus Salmonella within a randomly cloned sequence, a 559 bp target specific for Salmonella Typhimurium within the fliC gene and a 312 bp fragment specific for Salmonella Enteritidis within the sefA gene. The m‐PCR‐based assay was used for detecting Salmonella from 1078 environmental swabs of poultry houses. Prior to PCR, these swabs were pre‐enriched in phosphate‐buffered peptone water for 18–20 h and then sub‐cultured on a Modified Semi‐solid Rappaport Vassiliadis medium (MSRV) for 18–20 h. The m‐PCR combined with MSRV had a better sensitivity (95%) than the bacteriological method (92·5%). The MSRV‐m‐PCR assay and the bacteriological method had an agreement rate of 95·6%.