HUMAN MYELOMA CELLS AND THEIR STRONG STIMULATING CAPACITY IN ONE-WAY MIXED LYMPHOCYTE-REACTION - COMPARATIVE-STUDY WITH LEUKEMIC-B LYMPHOID-CELLS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (1) , 63-68
Abstract
Cultured human myeloma cells (ARH-77, RPMI-8226 and U-266), like leukemic B [bone marrow-derived] lymphoid cells, consistently exerted a strong stimulating capacity on allogeneic lymphocytes in the 1-way mixed lymphocyte reaction. Optimal stimulation was seen when a 1:1 ratio or 1:2 ratio of responding cell:stimulating cells of each cell line was utilized. Stimulating capacity of ARH-77 or RPMI-8226 cells significantly diminished when a 1:4 ratio of responding cells:stimulating cells was utilized. Fresh bone marrow cells containing more than 80% plasma cells from a patient with multiple myeloma failed to exert stimulating capacity on 2 occasions. The striking difference between cultured myeloma cells and fresh plasma cells is that the Ia[immune response-associated]-like antigen was present on cultured myeloma cells, and this antigen was absent on fresh plasma cells. The relationship between Ia-like antigen and stimulating capacity in 1-way mixed lymphocyte reaction was discussed.