Newcastle disease virus f glycoprotein expressed from a recombinant vaccinia virus vector protects chickens against live‐virus challenge

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Abstract
Chickens were immunised using a vaccinia recombinant virus (vaccinia‐Italien‐F), expressing the F protein of Newcastle disease virus (NDV). Immunisation was successful using either TK” cells infected with the vaccinia‐Italien‐F virus, the recombinant virus grown in TK7 cells and inoculated intra‐cerebrally in one‐day‐old chickens or the recombinant virus given by wing‐web to adult chickens after adaptation by alternate passage in chick embryo fibroblasts and chickens. The use of recombinant viruses expressing the F protein of NDV as vaccines would allow joint application of vaccination and eradication programmes for NDV. Therefore, recombinant viruses obtained in chickens virus vectors are needed.
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