Measuring the metabolome: current analytical technologies
Top Cited Papers
- 4 March 2005
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 130 (5) , 606-625
- https://doi.org/10.1039/b418288j
Abstract
The post-genomics era has brought with it ever increasing demands to observe and characterise variation within biological systems. This variation has been studied at the genomic (gene function), proteomic (protein regulation) and the metabolomic (small molecular weight metabolite) levels. Whilst genomics and proteomics are generally studied using microarrays (genomics) and 2D-gels or mass spectrometry (proteomics), the technique of choice is less obvious in the area of metabolomics. Much work has been published employing mass spectrometry, NMR spectroscopy and vibrational spectroscopic techniques, amongst others, for the study of variations within the metabolome in many animal, plant and microbial systems. This review discusses the advantages and disadvantages of each technique, putting the current status of the field of metabolomics in context, and providing examples of applications for each technique employed.Keywords
This publication has 230 references indexed in Scilit:
- Here is the evidence, now what is the hypothesis? The complementary roles of inductive and hypothesis‐driven science in the post‐genomic eraBioEssays, 2003
- Capillary electrophoresis – mass spectrometry: 15 years of developments and applicationsElectrophoresis, 2003
- Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT–IR and direct injection electrospray mass spectrometryComparative and Functional Genomics, 2003
- Monitoring of complex industrial bioprocesses for metabolite concentrations using modern spectroscopies and machine learning: Application to gibberellic acid productionBiotechnology & Bioengineering, 2002
- Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networksComparative and Functional Genomics, 2001
- Deconvolution gas chromatography/mass spectrometry of urinary organic acids - potential for pattern recognition and automated identification of metabolic disordersRapid Communications in Mass Spectrometry, 1999
- The Simultaneous Determination of Mixtures of Drug Candidates by Liquid Chromatography/Atmospheric Pressure Chemical Ionization Mass Spectrometry as anIn Vivo Drug Screening ProcedureRapid Communications in Mass Spectrometry, 1997
- A novel diagnostic test for arthritis: Multivariate analysis of infrared spectra of synovial fluidBiospectroscopy, 1997
- A new chemical diagnostic method for inborn errors of metabolism by mass spectrometry—rapid, practical, and simultaneous urinary metabolites analysisMass Spectrometry Reviews, 1996
- Analysis of rat urine for metabolites of pyrilamine via high-performance liquid chromatography/thermospray mass spectrometry and tandem mass spectrometryJournal of Mass Spectrometry, 1990