Detection of parvovirus B19 in donated blood: a model system for screening by polymerase chain reaction
- 1 February 1993
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 31 (2) , 323-8
- https://doi.org/10.1128/jcm.31.2.323-328.1993
Abstract
A highly sensitive and rapid method for routinely screening large numbers of donated blood units for parvovirus B19 by the polymerase chain reaction (PCR) was developed. Over a 3-month trial period in Edinburgh, B19 DNA was detected in 6 of 20,000 consecutive units of blood (0.03%), in concentrations ranging from 2.4 x 10(4) to 5 x 10(10) copies of viral DNA per ml. Seroconversion for B19-specific immunoglobulin M and immunoglobulin G and disappearance of circulating B19 DNA occurred in the interval between donation and recall in four of the five implicated donors who could be recalled. B19 DNA was detected in 18 of 27 separate batches of non-heat-treated factor VIII and IX concentrate manufactured from donated plasma unscreened for B19 DNA. Dry-heat treatment at 80 degrees C for 72 h reduced but did not always eliminate detectable B19 from factor VIII concentrates, consistent with recent observations that current methods for virus inactivation during blood product manufacture are insufficient to entirely eliminate B19 infectivity. The methods developed in this study for PCR screening could be applied routinely to prevent transfusion of B19 in blood and blood products and could play an important role in the prevention of iatrogenic transmission of infection. PCR screening could also be used for detection and exclusion of a range of other transmission-associated viruses for which current serological detection methods are only partially effective.Keywords
This publication has 25 references indexed in Scilit:
- Human parvovirus B19 infection in hemophiliacs first infused with two high‐purity, virally attenuated factor Vlll concentratesAmerican Journal of Hematology, 1992
- Parvovirus B19 for the hematologistAmerican Journal of Hematology, 1992
- Removal of parvovirus B19 from contaminated factor VIII during fractionationJournal of Medical Virology, 1991
- Genetic Diversity of Human Parvovirus B19 Determined Using a Set of Restriction Endonucleases Recognizing Four or Five Base Pairs and Partial Nucleotide Sequencing: Use of Sequence Variability in Virus ClassificationJournal of General Virology, 1991
- In vitro propagation of parvovirus B19 in primary foetal liver cultureJournal of General Virology, 1991
- Detection by PCR of hepatitis C virus in factor VIII concentratesThe Lancet, 1990
- Transmission of Human Parvovirus B19 by Coagulation Factor ConcentratesVox Sanguinis, 1990
- A New Peptide for Human Parvovirus B19 Antibody DetectionScandinavian Journal of Infectious Diseases, 1989
- Experimental Parvoviral Infection in HumansThe Journal of Infectious Diseases, 1985
- TRANSMISSION OF SERUM PARVOVIRUS-LIKE VIRUS BY CLOTTING-FACTOR CONCENTRATESThe Lancet, 1983