Human protein S cDNA encodes Phe‐16 and Tyr 222 in consensus sequences for the post‐translational processing

Abstract
Partial cDNAs coding for human protein S were isolated from a pUC9 human liver cDNA library. Together, the overlapping clones span a (partial) 5′‐non‐coding region, and the complete protein S coding and 3′‐untranslated regions. The derived amino acid sequence deviates at five positions from two previously reported protein S sequences. Two of these differences (Phe instead of Leu at position — 16 and Tyr instead of Asp at position 222) are found in regions that are important for the post‐translational modification of protein S, the γ‐carboxylation of glutamic acid and the hydroxylation of asparagine, respectively.