Comparison of Acridine Orange and Giemsa stains in several mouse bone marrow micronucleus assays—including a triple dose study

1 January 1989

journal article
research article
Published by Oxford University Press (OUP) in Mutagenesis

Vol. 4 (6) , 476-481
https://doi.org/10.1093/mutage/4.6.476

Abstract

Data from four mouse bone marrow micronucleus assays are presented in which sister slides were stained with Giemsa or Acridine Orange (AO). These data are used to support our decision to change from Giemsa stain to AO when conducting rodent bone marrow micronucleus assays. The AO method leads to definitive assessments of the incidence of micronucleated polychromatic erythrocytes on a slide, and in so doing, it saves reading time and increases confidence in data. Preliminary experiments with DMBA using the triple-dose/single sample protocol of MacGregor are also described, and it is concluded that this protocol shows great promise.

This publication has 10 references indexed in Scilit:

The effect of exposure regimen and duration on benzene-induced bone-marrow damage in mice
Mutation Research/Environmental Mutagenesis and Related Subjects, 1988
A simplified protocol for the mouse bone marrow micronucleus assay
Journal of Applied Toxicology, 1988
Re‐evaluation of 1,2‐dimethylhydrazine in the mouse bone marrow micronucleus assay: Observation of a positive response
Environmental Mutagenesis, 1987
Re‐evaluation of the need for multiple sampling times in the mouse bone marrow micronucleus assay: Results for DMBA
Environmental and Molecular Mutagenesis, 1987
Kinetics of micronucleus formation in relation to chromosomal aberrations in mouse bone marrow
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1984
Evaluation of some formaldehyde-release compounds and other biocides in the mouse micronucleus test
Mutation Research/Genetic Toxicology, 1983
A simple fluorescent staining procedure for micronuclei and RNA in erythrocytes using Hoechst 33258 and pyronin Y
Mutation Research Letters, 1983
An application of Acridine Orange fluorescent staining to the micronucleus test
Mutation Research Letters, 1983
Micronucleus test and bone-marrow chromosome analysis
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1981
The Micronucleus Test for Cytogenetic Analysis
Published by Springer Nature ,1976