Silica Gel Functionalised with Different Spacers as Solid Support for Oligonucleotide Synthesis

Abstract
The phosphotriester method for synthesis of oligonucleotides has been used for evaluating silica with spacer groups. Three different spacers were synthesized of which a long chain alkylamine, a double glycine and a double y-aminobutyric acid unit. Only the double glycine spacer improved the yields of the first condensations and is now routinely used in our laboratory.