An Escherichia coli expression–based method for heme substitution
- 24 December 2006
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 4 (1) , 43-45
- https://doi.org/10.1038/nmeth984
Abstract
Heme reconstitution with porphyrin analogs is a powerful approach toward understanding the molecular function of heme proteins; present methods, however, have not proven to be generally useful. Here we describe the development and application of an expression-based method for introducing modified porphyrins. The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions.Keywords
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