Association of Thy-1 differentiation alloantigen with synaptic complexes isolated from mouse brain.

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Abstract
Conventional fractionation procedures were used to define the subcellular distribution of the Thy-1 alloantigen in whole mouse brain. After discontinuous sucrose density gradient centrifugation of isotonic postnuclear particulate fractions, the bulk of Thy-1 was recovered in regions of the gradients containing synaptosomes. The synaptosome fraction that banded at 1.2 M sucrose yielded a specific activity for Thy-1 significantly greater than the synaptosomes separating at 1.4 M sucrose. Osmotic lysis of both synaptosome fractions resulted in further enrichment in Thy-1 activity with noconcomitant decrease in yields. The synaptosomal membranes obtained in this way were subsequently treated with Triton X-100 and subjected to further density gradient centrifugation. Although the detergent treatment resulted in some loss of antigenic activity, the gradient fractions that contained Thy-1 were found by EM to be richest in synaptic junctional complexes. Thy-1 may be associated with synaptosomes and synaptic junctional complexes and may be involved in the formation and/or maintenance of synaptic connections.