MACROPHAGE-LIKE CELL-TRANSFORMATION AND CFU(C) FLUCTUATIONS IN NORMAL AND LEUKEMIC HUMAN MARROW CULTURES TREATED BY PHORBOL DIESTER

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 31  (9-10) , 252-257
Abstract
Bone marrow from normal and chronic myeloid leukemia donors was grown in liquid cultures without feeder layers and with and without 12-O-tetradecanoylphorbol-13-acetate (TPA). In 24-96 h most of the cells (60-70%) cultured with 10-7 M and 10-8 M TPA stuck to the bottom of the flasks and had a peculiar shape resembling macrophages possessing strong phagocytizing activity and surface markers of monocytemacrophage lineage of differentiation. 10-7 M and 10-8 M TPA fully inhibited CFU (c) [agar colony-forming cells] in cultures of normal marrow and in that of chronic myeloid leukemia patients; 10-9 M and 10-10 M exhibited individually varied partial suppression. Cultivation of bone marrow with 10-11 M to 10-13 M TPA led in some cases to a statistically significant increase of CFU (c) on day 4 and day 7.