Isozyme Banding Patterns in Two Stands of Cetraria arenaria Kärnef.

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Abstract
An attempt was made to explore the range of isozymes detectable in 2 stands of C. arenaria Karnef. established in stabilized sand dunes along the eastern shore of Lake Huron, [Canda] Proteins in crude extracts were separated by isoelectric focusing and tests were made for peroxidase, catalase, esterase, carbonic anhydrase, acid phosphatase, alkaline phosphatase, lactate dehydrogenease, malate dehydrogenase and glutamate dehydrogenase activity. Some gels were also stained with Coumassie Brilliant Blue, a general protein stain. Storage of acetone-extracted thallus pieces at -20.degree. C for as long as 10-12 mo. had little effect on isozyme banding patterns. Most of the bands appeared to be common among samples collected in the same stand, but some notable within-stand differences were observed with respect to esterases and acid and alkaline phosphatases. Only minor quantitative differences in proteins were observed between the 2 stands.