Cloning of two β‐tubulin related genes in tetrahymena pyriformis
- 2 September 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 188 (2) , 227-232
- https://doi.org/10.1016/0014-5793(85)80377-x
Abstract
Genomic clones containing β‐tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin‐related genes has been established by cross‐hybridization with a cDNA containing a complete β‐tubulin coding region from Chlamydomonas and by the positive hybrid‐selected translation assay. The respective tubulin‐related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β‐tubulin‐related genes are physically linked and clustered in the genome.Keywords
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