SYNERGISTIC KILLING EFFECT OF GLYCINE AND PENICILLIN, AND RAPID METHODS FOR MUTANT ISOLATION IN PSEUDOMONAS
- 1 January 1967
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 13 (2) , 205-215
- https://doi.org/10.2323/jgam.13.205
Abstract
The addition of 3% glycine and 10 units/ml of penicllin-G to a culture of Pseudomonas resulted in rapid death. More than 95% of viable cells were killed within 30 min of incubation. Cell wall synthesis, as determined byamino-acid incorporation, was inhibited by glycine plus penicillin. Penicillin in high concentrations induced some kind of unbalanced growth, while EDTA which had little or no effect on intact cells or cells treated with penicillin in a concentration of 1x10-4[image] lysed cells treated with glycine and penicillin. The combined action of these chemicals was utilized for the rapid screening of temperature-sensitive mutants from UV-irradiated cells. N-Nitroso-N-methylurethane was an extremely efficient mutagen for the isolation of temperature-sensitive mutants. The frequency of mutation was so high that mutants can be isolated without any screening procedure.This publication has 4 references indexed in Scilit:
- Glycopeptide transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions.Proceedings of the National Academy of Sciences, 1966
- Penicillin: its basic site of action as an inhibitor of a peptide cross-linking reaction in cell wall mucopeptide synthesis.Proceedings of the National Academy of Sciences, 1965
- A NONSPECIFIC INCREASE IN PERMEABILITY IN ESCHERICHIA COLI PRODUCED BY EDTAProceedings of the National Academy of Sciences, 1965
- The lethal and mutagenic effects of N-nitroso-N-methyl-urethane and N-nitroso-N-ethylurethane in Colletotrichum coccodesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1964