Efficient incorporation of unnatural amino acids into proteins in Escherichia coli
- 22 March 2006
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 3 (4) , 263-265
- https://doi.org/10.1038/nmeth864
Abstract
We have developed a single-plasmid system for the efficient bacterial expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNATyrCUA) are expressed under control of the proK promoter and terminator, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expressed under control of a mutant glnS (glnS') promoter.Keywords
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