DETECTION OF MITOGEN-ACTIVATED T AND NON-T LYMPHOCYTES BY VIRUS PLAQUE ASSAY - VIRUS PLAQUE ASSAY ON CELLS FRACTIONATED BY UNIT GRAVITY SEDIMENTATION

  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (6) , 875-883
Abstract
Virus plaque assay (VPA) was utilized for the quantitative evalution of activated [mouse] lymphocytes. An examination was made of what types of cells, especially which of activated T [thumus-derived] and non-T lymphocytes, were detected as infective centers after infection with vesicular stomatitis virus. Marked increases in DNA synthesis and in virus-plaque forming cells (V-PFC) were observed not only during the activation of T lymphocytes with Con A [concanavalin A], but also, though to a lesser extent, during the activation with [Salmonella typhimurium] lipopolysaccharide (LPS) of non-T lymphocyte preparations of nude spleen from which .theta.-positive lymphocytes and macrophages were completely depleted. The latter observation was further confirmed by the VPA on the populations enriched in LPS-activated non-T lymphocytes fractionated by the unit gravity sedimentation method. Fast sedimenting cells were more active in DNA synthesis and contained more infective centers after infection than those sedimenting slowly and original unfractionated cells. The capacity for DNA synthesis and virus-replication were considered to be general properties accompanying lymphocyte activation.