Residence time effects on monoclonal antibody binding to adsorbed fibrinogen

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Abstract
Fibrinogen adsorbed to polymeric surfaces and then allowed to reside on the surface while it is kept in a buffer solution for a period of time (the 'residence time') undergoes postadsorptive changes that decrease its SDS elutability, displaceability by plasma, polyclonal antifibrinogen binding, and ability to support platelet adhesion (summarized in Chinn et al. J. Biomed. Mater. Res. 26, 757 (1992)). In order to better understand the nature of the changes in adsorbed fibrinogen, the binding of ten different monoclonal antifibrinogen molecules to fibrinogen adsorbed from plasma to Biomer and several other surfaces has been measured after increasing residence time in buffer. Three of the monoclonal antibodies used bind to sequences that have been implicated in platelet binding to fibrinogen. One of these (M1) binds to the C-terminal region of the gamma chain (402-411), another (R1) binds to the N-terminal region of the A alpha chain containing an RGDF sequence (95-98), and the third (R2) binds to the C-ter...

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