Tryptophan photoproduct(s): sensitized induction of strand breaks (or alkali-labile bonds) in bacterial deoxyribonucleic acid during near-ultraviolet irradiation
- 1 April 1975
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 122 (1) , 199-205
- https://doi.org/10.1128/jb.122.1.199-205.1975
Abstract
Long-wavelength ultraviolet light (300 to 400 nm) converts L-tryptophan to a photoproduct that is toxic for bacterial cells in dark conditions. We now report that similar photoproducts of l-tryptophan sensitize bacterial deoxyribonucleic acid to 365-nm radiation, increasing the yield of deoxyribonucleic acid strand breaks (or alkali-labile bonds) by approximately 11.5-fold. Evidence is also presented which indicates that thse sensitized deoxyribonucleic acid lesions contribute to lethality for Escherichia coli irradiated with 365-nm ultraviolet light in suspensions of tryptophan photoproducts.Keywords
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