Flavin 1, N 6 -Ethenoadenine Dinucleotide: Dynamic and Static Quenching of Fluorescence
Open Access
- 1 March 1973
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (3) , 941-943
- https://doi.org/10.1073/pnas.70.3.941
Abstract
Flavin 1,N6-ethenoadenine dinucleotide (εFAD) was prepared by the action of chloroacetaldehyde on flavin adenine dinucleotide. This compound, which has two potential fluorophores, ε-adenine and isoalloxazine, shows extremely efficient energy transfer from the former to the latter. The fluorescences of both moieties are greatly diminished in the intact molecule. Determination of the fluorescence yields and lifetimes leads to the conclusion that at 20° in neutral aqueous solution εFAD exists mainly (90%) as an internally complexed or stacked form.Keywords
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