Hemoglobins, XXXVIII. Amino Acid Sequence of a Dimeric Hemoglobin (Erythrocruorin), Component VI fromChironomus thummi thummi(CTT VI)

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Abstract
The dimeric hemoglobin CTT VI (Erythrocruorin) was isolated from the hemolymph of the larvae of C. thummi thummi. The globin from CTT VI was subjected to trypsin, limited trypsin and cyanogen bromide digestion. For elucidation of the sequence in the C-terminal region, cleavage with Staphylococcus aureus V8 protease was carried out. The peptides were separated by gel and ion-exchange chromatography. The handling of some large fragments was facilitated by maleylation and subsequent ion-exchange chromatography with conservation of the maleyl groups. The amino acid sequence was determined by automatic Edman degradation. The order of the peptides was provided by overlaps, but in 2 cases by homology only (for residues 98 and 99, and 109 and 110). The hemoglobin consists of 2 .times. 147 amino acids with a MW of 32,411. The sequence of CTT VI is compared with a monomeric (CTT III) and a dimeric hemoglobin (CTT II.beta.) and the human .alpha.-chains. The structural differences are discussed.

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