Radioimmunoassay for an Inhibin-Like Peptide from Human Seminal Plasma
- 6 December 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 6 (4) , 363-369
- https://doi.org/10.1080/01971528508063039
Abstract
Antisera raised in rabbits to a synthetic peptide consisting of 31 amino acids with a sequence identical to inhibin-like peptide (ILP) isolated from human seminal plasma afford a highly specific and sensitive radioimmunoassay. Synthetic ILP completely displaces antiserum binding of radioiodinated [Tyr4]-ILP, with half maximal displacement at 36 fmoles ILP/tube. ILP, [Tyr4]-ILP and ILP-(9–31) had essentially equal potency, while ILP-(l-25), ILP-(l-23) and ILP-(1–16) had reduced potency. No cross reactivity was found among a variety of peptide hormones and proteins. Human seminal plasma displaces 50% of [125I-Tyr4]-ILP at dilutions equivalent to 50–250 pl/tube, corresponding to immunoreactive ILP concentration of 0.5–2.5 mg/ml.Keywords
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