High Performance Chromatography of Amino Acids, Peptides and Proteins XXIII · Peptide Mapping by Hydrophilic Ion-paired, Reversed-Phase High Performance Liquid Chromatography for the Characterisation of the Tryptic Digest of Haemoglobin Variants
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 4 (4) , 599-612
- https://doi.org/10.1080/01483918108059958
Abstract
The application of high performance liquid chromatography on reversed-phase columns for the tryptic mapping of haemoglobin variants is reported. The effect of flow rate and gradient shape on resolution and column efficiencies has been examined using acetonitrile-water-orthophosphoric acid elution systems. With these conditions it is possible to recognise sequence changes for variant haemoglobins including HbC, HbS, HbE, HbJ Cambridge and HbD Punjab.This publication has 15 references indexed in Scilit:
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