Plasmid DNA Supercoiling and Gyrase Activity in Escherichia coli Wild-Type and rpoS Stationary-Phase Cells
- 1 February 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (3) , 1097-1100
- https://doi.org/10.1128/jb.185.3.1097-1100.2003
Abstract
Stationary-phase cells displayed a distribution of relaxed plasmids and had the ability to recover plasmid supercoiling as soon as nutrients became available. Preexisting gyrase molecules in these cells were responsible for this recovery. Stationary-phase rpoS cells showed a bimodal distribution of plasmids and failed to supercoil plasmids after the addition of nutrients, suggesting that rpoS plays a role in the regulation of plasmid topology during the stationary phase.Keywords
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