Fast nonlinear spectral microscopy of in vivo human skin
Open Access
- 18 January 2011
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 2 (2) , 365-373
- https://doi.org/10.1364/boe.2.000365
Abstract
An optimized system for fast, high-resolution spectral imaging of in vivo human skin is developed and evaluated. The spectrograph is composed of a dispersive prism in combination with an electron multiplying CCD camera. Spectra of autofluorescence and second harmonic generation (SHG) are acquired at a rate of 8 kHz and spectral images within seconds. Image quality is significantly enhanced by the simultaneous recording of background spectra. In vivo spectral images of 224 × 224 pixels were acquired, background corrected and previewed in real RGB color in 6.5 seconds. A clear increase in melanin content in deeper epidermal layers in in vivo human skin was observed.Keywords
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