The Cellophane Technique for Studying Morphology and Hyphal Fusions in Fungi

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Abstract
Morphology and hyphal fusions can be studied readily if fungi are grown on small, sterile, squares of cellophane placed on the surface of suitable agar media. Colonies on cellophane may be mounted in lactophenol or lacto-fuchsin for microscopic examination. The technique yields many of the observational advantages of a microculture, but requires no more time and effort than an ordinary culture and mount.