Effect of Secondary Structure of Messenger Ribonucleic Acid on the Formation of lnitiation Complexes with Prokaryotic and Eukaryotic Ribosomes

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Abstract
The effect of modification of the secondary structure of phage f2 RNA and brome mosaic virus (BMV) RNA 3 on the formation of initiation complexes in Escherichia coli and wheat germ protein-synthesizing systems was studied. Modification of the RNAs was achieved by using O-methylhydroxylamine, which specifically reacts with cytosines; this leaves the initiation codons unchanged and, under denaturing conditions, leads to irreversible unfolding of the RNA. E. coli ribosomes interact with newly exposed AUG/GUG codons in the modified templates forming polysomes, whereas they form monosomes with native f2 RNA or BMV RNA 3. With wheat germ ribosomes, disomes are formed in the presence of BMV RNA 3, native or modified. With f2 RNA, eukaryotic ribosomes form monosomes, independent of the secondary structure of the template. In contrast to prokaryotic ribosomes, binding of eukaryotic ribosomes to f2 RNA of BMV RNA 3 is not affected by modification of the secondary structure of these messengers.