Inactivation of Synchronized Chinese Hamster V79 Cells with Charged-Particle Track Segments

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Abstract
Synchronized Chinese hamster V79 [lung] cells were irradiated with protons, deuterons and 3He ions in a series of cell inactivation experiments. The charged particles had a range of LET [linear energy transfer] values from 10-170 keV/.mu.m. Two parts of the cell cycle were irradiated to determine clonogenic survival, the G1/S transition and late-S phase. G1/S transition and late-S phase were obtained by hydroxyurea-induced synchrony. The difference in radiation sensitivity between these 2 synchronized cell populations decreased with increased values of LET, but a 10-fold difference persisted with the highest LET values. The inactivation cross sections calculated from initial slopes of the survival curves increased to plateau values of about 19 and 38 .mu.m2 for the late-S and G1/S cells, respectively. The respective cross-sections of cell nuclei were measured and had mean values of 222 and 165 .mu.m2.