Interaction and Regulation of Subcellular Localization of CED-4 by CED-9
- 21 February 1997
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 275 (5303) , 1126-1129
- https://doi.org/10.1126/science.275.5303.1126
Abstract
The Caenorhabditis elegans survival gene ced-9 regulates ced-4 activity and inhibits cell death, but the mechanism by which this occurs is unknown. Through a genetic screen for CED-4-binding proteins, CED-9 was identified as an interacting partner of CED-4. CED-9, but not loss-of-function mutants, associated specifically with CED-4 in yeast or mammalian cells. The CED-9 protein localized primarily to intracellular membranes and the perinuclear region, whereas CED-4 was distributed in the cytosol. Expression of CED-9, but not a mutant lacking the carboxy-terminal hydrophobic domain, targeted CED-4 from the cytosol to intracellular membranes in mammalian cells. Thus, the actions of CED-4 and CED-9 are directly linked, which could provide the basis for the regulation of programmed cell death in C. elegans.Keywords
This publication has 23 references indexed in Scilit:
- Bax Can Antagonize Bcl-XL during Etoposide and Cisplatin-induced Cell Death Independently of Its Heterodimerization with Bcl-XLJournal of Biological Chemistry, 1996
- An Alternatively Spliced C. elegans ced-4 RNA Encodes a Novel Cell Death InhibitorCell, 1996
- Molecular Ordering of the Cell Death Pathway: Bcl-2 AND Bcl-xL FUNCTION UPSTREAM OF THE CED-3-LIKE APOPTOTIC PROTEASESPublished by Elsevier ,1996
- Developing Caenorhabditis elegans neurons may contain both cell-death protective and killer activities.Genes & Development, 1996
- The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzymeCell, 1993
- bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell deathCell, 1993
- Prevention of Programmed Cell Death in Caenorhabditis elegans by Human bcl-2Science, 1992
- Mechanisms and Functions of Cell DeathAnnual Review of Cell Biology, 1991
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Mutations Affecting Programmed Cell Deaths in the Nematode Caenorhabditis elegans sScience, 1983