Visualization of pronuclei in living bovine zygotes
- 1 April 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 30 (3) , 687-691
- https://doi.org/10.1095/biolreprod30.3.687
Abstract
Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one-celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei were visualized, identified and subjected to micromanipulation.This publication has 14 references indexed in Scilit:
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