High fidelity extrachromosomal recombination and gene targeting in plants
- 1 January 1994
- journal article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 243 (1) , 106-111
- https://doi.org/10.1007/bf00283882
Abstract
The precision of extrachromosomal homologous recombination and gene targeting in plant cells was investigated. Recombination was directed to introns of selectable marker genes where potential changes could persist without affecting the function and therefore the selectability of the genes. Approximately 9 kb of crossover regions was rescued and sequenced. Changes were detected at a frequency below one point mutation per 1000 bp, indicating that extrachromosomal recombination and gene targeting both appear to occur with high fidelity.Keywords
This publication has 42 references indexed in Scilit:
- Homologous recombination in plant cells after Agrobacterium-mediated transformation.Plant Cell, 1990
- HOMOLOGOUS RECOMBINATION IN MAMMALIAN CELLSAnnual Review of Genetics, 1989
- The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicingCell, 1989
- Fidelity of DNA synthesis by the Thermus aquaticus DNA polymeraseBiochemistry, 1988
- Recombination of Selectable Marker DNA inNicotiana tabacumDNA, 1987
- Disruption of the Dictyostelium Myosin Heavy Chain Gene by Homologous RecombinationScience, 1987
- Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate geneNature, 1986
- High frequency targeting of genes to specific sites in the mammalian genomeCell, 1986
- Insertion of DNA sequences into the human chromosomal β-globin locus by homologous recombinationNature, 1985
- Direct gene transferState of the Art and Future PotentialPlant Molecular Biology Reporter, 1985