Transposon Tn3 encodes a site-specific recombination system: identification of essential sequences, genes, and actual site of recombination.
- 1 July 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (7) , 4041-4045
- https://doi.org/10.1073/pnas.78.7.4041
Abstract
The bacterial transposon Tn3 encodes a site-specific recombination system. The recombination requires the product of tnpR, a gene previously identified as a repressor of the transposase. This recombination is site specific and takes place somewhere within the sequence C-G-A-A-A-T-A-T-T-A-T-A-A-A-T-T-A-T-C but requires at least 1 additional sequence outside this. The phenotype of mutations in this recombination system suggests that transposition proceeds by a mechanism in which cointegrates are intermediates.Keywords
This publication has 21 references indexed in Scilit:
- Phase Variation: Evolution of a Controlling ElementScience, 1980
- Site-specific recombination functions of bacteriophage λ: DNA sequence of regulatory regions and overlapping structural genes for Int and XisProceedings of the National Academy of Sciences, 1980
- DNA sequence analysis of the transposon Tn3: Three genes and three sites involved in transposition of Tn3Cell, 1979
- Transposition protein of Tn3: identification and characterisation of an essential represser-controlled gene productNature, 1979
- Transposition of the Escherichia coli insertion element gamma generates a five-base-pair repeat.Proceedings of the National Academy of Sciences, 1979
- Dissection of the transposition process: A transposon-encoded site-specific recombination systemMolecular Genetics and Genomics, 1979
- Identification and characterization of a self-regulated repressor of translocation of the Tn3 element.Proceedings of the National Academy of Sciences, 1979
- Molecular model for the transposition and replication of bacteriophage Mu and other transposable elements.Proceedings of the National Academy of Sciences, 1979
- Nucleotide-sequence Analysis of Tn3 (Ap): Implications for Insertion and DeletionPublished by Cold Spring Harbor Laboratory ,1979
- Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322Published by Cold Spring Harbor Laboratory ,1979