Phosphorylation ofToxoplasma gondii major surface antigens
- 1 January 1992
- journal article
- research article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 78 (7) , 541-544
- https://doi.org/10.1007/bf00936449
Abstract
The phosphorylation of the major surface proteins ofToxoplasma gondii tachyzoites was investigated. Metabolic labeling of intracellular tachyzoites with [32P]-orthophosphate followed by immunoprecipitation with specific monoclonal antibodies showed that all five major surface proteins were labeled as expected for GPI-anchored proteins. More detailed analysis of the phosphorylated sites revealed that surface proteins P30 and P22 contained phosphoserine residues in addition to the phosphorylated molecules that are presumably localized in the GPI-membrane anchorsKeywords
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