The isolation and properties of a clonal tissue culture strain of pluripotent mouse teratoma cells

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Abstract
A clonal tissue culture strain of pluripotent cells has been isolated from a transplantable teratoma of inbred strain of mice 129 Sv-S1j CP. This cell strain SIKR when re-inoculated into mice produces teratomas containing at least ten types of tissue. Sub-clones have been isolated and two types distinguished. (1) ‘C-type’ with a densely-piled in vitro growth. These are tumourigenetic and pluripotent displaying a comparable range of differentiation to the original SIKR. (2) ‘E-type’ spreading, often epithelioid growth. These grow to a lower density in culture than ‘C-type’. Mostly non-tumourigenetic; in those cases where a tumour has been obtained it did not display multiple differentiations. The results are interpreted as demonstrating that the culture consists of equivalently pluripotent cells which may become determined and differentiate spontaneously in vitro into slower growing cell types which are continuously overgrown by the culture.