H2‐forming N5,N10‐methylenetetrahydromethanopterin dehydrogenase: mechanism of H2 formation analyzed using hydrogen isotopes
- 17 July 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 368 (2) , 203-206
- https://doi.org/10.1016/0014-5793(95)00642-m
Abstract
H2‐forming N 5,N 10‐methylenetetrahydromethanopterin dehydrogenase catalyzes the reversible dehydrogenation of N 5,N 10‐methylenetetrahydromethanopterin (CH2 = H4MPT) to N 5,N 10‐methenyltetrahydromethanopterin (CH = H4MPT+) and H2. In D2O both HD and D2 are formed from CH2 = H4MPT and in H2O both HD and H2 from CD2 = H4MPT. Evidence is presented that HD is not an intermediate in the formation of D2 and H2, respectively.Keywords
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