STUDIES ON PEPTIDES .113. SYNTHESIS OF THE HEPTACOSAPEPTIDE AMIDE CORRESPONDING TO THE ENTIRE AMINO-ACID-SEQUENCE OF CHICKEN SECRETIN

  • 1 January 1984
    • journal article
    • research article
    • Vol. 23  (2) , 174-186
Abstract
The heptacosapeptide amide corresponding to the entire amino acid sequence of chicken secretin was synthesized by assembling 4 peptide fragments followed by deprotection with thioanisole-mediated trifluoromethanesulfonic acid in trifluoroacetate. The deprotected peptide was purified by gel-filtration on Sephadex G-25, followed by ion-exchange chromatographies on carboxymethyl-cellulose and DEAE-Sepharose. Synthetic peptide stimulated the flow of pancreatic alkaline juice and decreased systemic blood pressure in rats.

This publication has 11 references indexed in Scilit: